Updated Molecular Diagnosis of Chronic Hepatitis C

Abousree T Ellethy, Hamdy A Sliem, Gamal MA Hassan


The scope of this review allows an exhaustive analysis of updated molecular diagnosis of hepatitis C in endemic estimates representing examples to illustrate the use of molecular techniques for the HCV diagnosis. Hepatitis C is an emerging infection that threatens for economic and psychosocial disaster in estimated endemic areas worldwide. It is a global Human calamity requiring integrity of all researchers' efforts with strong governmental aids. Findings of current review article focused on the utility of measuring HCV core antigen levels in the clinical setting, as a substitution and/or conformational for measuring HCV RNA levels, especially in endemic areas with low economics. Currently, molecular assays based RT PCR allows the monitoring and generation of PCR products simultaneous with the amplification process. Quantitative assessment of HCV RNA levels, via signal amplification and quantitative PCR, are valuable tools in the clinical management of patients before, during and after therapy. Fortunately, the most promising approach is fully automated real-time PCR assays, which are faster, more sensitive than classical target amplification techniques and are not prone to carryover contamination.


Laboratory diagnosis; Hepatitis C

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