TGF-β1 (C-509T) Gene Promoter Polymorphism and its Influence on Chronic Pancreatitis

Kavutharapu Sri Manjari, Akka Jyothy, Porika Shravan Kumar, Boddu Prabhakar, Prathibha Nallari, Ananthapur Venkateshwari


AIM: Chronic pancreatitis is a long-standing inflammation of the pancreas that alters its normal structure and function. The aim of the study is to investigate the role of promoter polymorphism in the TGF-β1-509 (C/T) gene in chronic pancreatitis. METHODS: A total of 100 chronic pancreatitis patients and 100 control subjects were included in the present study. The genotyping of promoter polymorphism in the TGF-β1-509 (C/T) gene was carried out by tetra-primer ARMS PCR followed by agarose gel electrophoresis. The serum TGF-β1 levels were measured by enzyme-linked immunosorbent assay. Statistical analysis was applied for the significance of the results. RESULTS: The genotype distribution was 57% of C/C, 38% of C/T and 5% of T/T in control subjects, whereas in chronic pancreatitis patients they were 37% of C/C, 39% of C/T, and 24% of T/T genotype respectively. A significant variation in the distribution of T allele was observed in the patient group compared to control subjects [OD = 0.410 (0.261-0.643), p<0.000**]. There was a significantly higher levels of TGF-β1 levels in CP patients compared to healthy subjects. CONCLUSION: The present study found a significant association between the TGF-β1-509 (C/T) gene promoter polymorphism and chronic pancreatitis. Further, the levels were also found to be elevated in patients compared to control subjects. Thus, TGF-β1-509 (C/T) genotype can be considered as one of the risk factor in the etiology of the disease.


Chronic inflammation; Pancreatic fibrosis; Extra cellular matrix; Cytokines; Polymorphism

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