A New Approach to Analyzing Endophytic Actinobacterial Population in the Roots of Banana Plants (Musa sp., AAA)

Yanyan Zhai, Wenfeng Wang, Hongming Tan, Lixiang Cao


AIM: To elucidate the endophytic actinobacterial populations of healthy banana plant roots.

METHODS: A surface sterilization protocol was adopted to remove rhizoplane bacterial DNAs and total DNAs of surface-sterilized roots were purified and analyzed by Illumina-based sequencing.

RESULTS and CONCLUSION: A total of 2152102 bacterial sequences remained with an average length of 448bp, 31952 different actinobacterial OTUs at the 97% similarity level were obtained, and the dominant families were Nocardioidaceae, PseudoNocardiaceae and Nocardiaceae, which accounted for 56.37%, 14.36% and 9.77%, respectively. At genus level, Pseudonocardia (11.83%), Rhodococcus (9.74%), Nocardioides (3.06%) were the most abundant taxa. The results indicated that surface sterilization approach with formaldehyde (36%) and Illumina-based sequencing is suitable for analysis of endophytic actinobacterial populations in banana roots.


Banana roots; Endophytic actinobacterial; Surface-sterilization; Illumina-based analysis

Full Text: PDF HTML


  • There are currently no refbacks.